The Impact of Suggested Dosage Percentage of NPK Fertilizer and Biofertilizer on the Growth and Production of Inpari 32 Rice Variety (Oryza sativa L.)
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Abstract
The study was conducted at the Agricultural, Fisheries, and Forestry Extension Agency (BP3K) in Cilamaya Wetan District, Karawang Regency, West Java, at an elevation of 8.9 m above sea level, from December 2022 to March 2023.
Aims. The research focuses on exploring how the recommended percentage of NPK fertilizer interacts with biological fertilizer to affect the growth and yield of the lowland rice variety Inpari 32 and to determine the ideal biological fertilizer for each NPK dose level that maximizes grain weight per plot.
Methods. The experimental design used was a factorial pattern, a Randomized Block Design with two factors and repeated three times. The first factor is the dose of NPK fertilizer (N), which includes three treatment levels, namely: n1 = 100% of the suggested dose (500 g plot-1); n2 = 50% of the suggested dose (250 g plot-1), and n3 = 25% of the suggested dose (125 g plot-1). The second factor is the biological fertilizer dose (H), which includes 3 treatment levels, namely: h1 = 0% of the recommended dose (0 g plot-1); h2 = 50% of the recommended dose (15 g plot-1), and h3 = 100% of the recommended dose (30 g plot-1).
Result. The experimental results indicated an interaction between the recommended percentage of NPK fertilizer and biological fertilizer regarding plant height and number of tillers aged 28 Days After Planting (DAP), 42 DAP, and 56 DAP, along with the number of productive tillers, panicle length, grain weight per panicle, grain weight per clump, and grain weight per plot. The highest grain weight per plot of 24.32 kg was attained at the advised NPK fertilizer dosage of 100%, along with the ideal recommended biological fertilizer dose of 66,10% (r2 = 0.92).
Conclusion. The suggested NPK fertilizer dosage of 50% yielded the highest grain weight per plot of 23.77 kg, using the ideal recommended biological fertilizer amount of 60.19% (r2 = 0.90).
Implication. With a suggested application of NPK fertilizer of 25%, the highest grain mass per plot of 23.15 kg at an ideal biological fertilization rate of 55.10% (r2 = 0.94).
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References
Afifah, Z., Carsono, N., Sari, S., & Anas, A. 2020. Yield Testing and Selection of F4 and F6 Generation Rice Families from Crosses of Sintanur x PTB 33 and Pandanwangi x PTB 33 in Jatinangor. Agrosainstek: Journal of Agricultural Science and Technology, 4(1), 28–34. https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/96.
https://doi.org/10.33019/agrosainstek.v4i1.96 DOI: https://doi.org/10.33019/agrosainstek.v4i1.96
Azmi, Y., B.S. Purwoko, I.S. Dewi, M. Syukur, and T. Suhartini. 2017. Anther Culture of Crossbreeding of Local Black Rice with Cultivated Varieties (Fatmawati and Inpari 13). J. Agron. Indonesia, December 2017, 45(3):228-234. ISSN 2085-2916 e-ISSN 2337-3652
http://jai.ipb.ac.id. DOI: https://dx.doi.org/10.24831/jai.v45i3.12544 DOI: https://doi.org/10.24831/jai.v45i3.12544
Akbar, M., Fauzi, Asis, Asis, Lizmah, Fitria, & Sumeinika. 2022. Relationship between Agronomic Characteristics of Ciherang and Inpari 32 Rice Varieties in Rainfed Lowland Areas. Jurnal Agrium, 19(1):29. https://ojs.unimal.ac.id/agrium/article/view/6764. https://doi.org/10.29103/agrium.v19i1.6764 DOI: https://doi.org/10.29103/agrium.v19i1.6764
Akbar, M. R., Purwoko, B. S., Dewi, I. S., Suwarno, W. B., & Sugiyanta, D. 2019. Determination of Selection Index for High-Yielding Rainfed Lowland Rice Dihaploid Lines. Indonesian Journal of Agronomy, 47(2): 111–118. https://doi.org/10.24831/jai.v47i2.25032 DOI: https://doi.org/10.24831/jai.v47i2.25032
Anshori, M. F., Purwoko, B. S., Dewi, I. S., Ardie, S. W., Suwarno, W. B., & Safitri, D. H. 2018. Heritability, Characterization, and Clustergram Analysis of Dihaploid Rice Lines Resulting from Anther Culture. Indonesian Journal of Agronomy, 46(2): 119. https://doi.org/10.24831/jai.v46i2.18377 DOI: https://doi.org/10.24831/jai.v46i2.18377
Carsono, N., Lidiasari, D., Sari, S., & Wicaksana, N. 2022. Use of Natural and Synthetic Putrescine for Callus Induction and Anther Culture Regeneration of Four Rice Genotypes. Agriculture, 32(3): 314. https://doi.org/10.24198/agrikultura.v32i3.36784 DOI: https://doi.org/10.24198/agrikultura.v32i3.36784
Dewi, I.S., S. Azmy, B.S. Purwoko. 2024. Callus induction and plant regeneration from anther culture of gamma rays irradiated rice. AIP Conf. Proc. 2957, 080017 (2024). https://doi.org/10.1063/5.0183941. https://pubs.aip.org/aip/acp/article-abstract/2957/1/080017/3262246/Callus-induction-and-plant-regeneration-from DOI: https://doi.org/10.1063/5.0183941
Feng, Zhizun, Huangwei Chu, Liming Cao, Ruiyun Wang, Anpeng Zhang. 2026. A Review of Research Progress in Rice Anther Culture. Molecular Plant Sciences, Mol. Biol. 2026 48(1):18. https://www.mdpi.com/1467-3045/48/1/18. https://doi.org/10.3390/cimb48010018. DOI: https://doi.org/10.3390/cimb48010018
Gunarsih C., B.S. Purwoko, I.S. Dewi, W.B. Suwarno, & Nafisah. 2022. Evaluation of Dihaploid Lowland Rice Lines Resulting from Anther Culture Using Index Selection. Indonesian Journal of Agronomy, 50(1):18–25.
https://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/39898. https://doi.org/10.24831/jai.v50i1.39898 DOI: https://doi.org/10.24831/jai.v50i1.39898
Iriani, Y.F., D.C. Prayantini, H.N. Nahampun, R. Mastuti, N. Harijati. 2025. The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.). Journal of Science Education Research 11(8): 38-45. DOI: 10.29303/jppipa.v11i8.11884. https://jppipa.unram.ac.id/index.php/jppipa/article/view/11884 DOI: https://doi.org/10.29303/jppipa.v11i8.11884
Lantos, C., M. Jansco, A. Szekely, E. Nagy, T. Szaloki and J. Pauk. 2022. Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rize (Oryza sativa L.) Breeding. Plants 11(24), 3446. https://doi.org/10.3390/plants11243446. https://www.mdpi.com/2223-774 DOI: https://doi.org/10.3390/plants11243446
Linton, J. D., Klassen, R., Jayaraman, V., Walker, H., Brammer, S., Ruparathna, R., Hewage, K., Thomson, J., Jackson, T., Baloi, D., Cooper, D. R., Hoejmose, S. U., Adrien-Kirby, A. J., Sierra, L. A., Pellicer, E., Yepes, V., Giunipero, L. C., Hooker, R. E., Denslow, D., Anane, A. (2020). Agronomic Performance and Yield Potential of Several Dihaploid Rice (Oryza sativa L.) Lines from Anther Culture in Karawang Agronomic Regency. Sustainability (Switzerland), 14(2) : 1–4. http://www.unpcdc.org/media/15782/sustainable procurement practice.pdf
Mawaddah, Purwoko, B. S., Dewi, I. S., & Wirnas, D. D. 2018. Characterization of Agronomic Traits of Dihaploid Red Rice Plants with High Yield Potential Obtained through Anther Culture. Indonesian Journal of Agronomy, 46(2): 126-132. P-ISSN: 2085-2916, E-ISSN: 2337-3652. https://doi.org/10.24831/jai.v46i2.16249 DOI: https://doi.org/10.24831/jai.v46i2.16249
Firdaus, M.F., Bambang Sapta Purwoko, Iswari Saraswati Dewi, & Willy Bayuardi Suwarno. 2022. Physicochemical Characterization of Dihaploid Black Rice Lines. Indonesian Journal of Agronomy, 50(1): 1–9. https://doi.org/10.24831/jai.v50i1.39850 DOI: https://doi.org/10.24831/jai.v50i1.39850
Prasetia, A. A., Jazilah, S., & Badrudin, U. 2022. The Effect of Planting Systems on the Growth and Production of Several Rice Varieties (Oryza sativa L.). Biofarm: Agricultural Scientific Journal, 18(1): 53.
https://doi.org/10.31941/biofarm.v18i1.1887 DOI: https://doi.org/10.31941/biofarm.v18i1.1887
Romero, F.M. and A.G. Arias. 2019. CRISPR/Cas9: Development and Application in Rice Breeding. Rice Science 26(5): 265–281. ScienceDirect. Elsevier. https://www.sciencedirect.com/science/article/pii/S1672630819300599?via%3Dihub
https://doi.org/10.1016/j.rsci.2019.08.001 DOI: https://doi.org/10.1016/j.rsci.2019.08.001
Safitri, H., B. Purwoko, I. Dewi, S.W. Ardie. 2016. Anther Culture to Obtain Salinity-Tolerant Rice Lines. J. Agron. Indonesia 44 (3): 221–227. https://www.researchgate.net/publication/316205586_Kultur_Antera_untuk_Mendapatkan_Galur_Padi_Toleran_Salinitas. DOI: 10.24831/jai.v44i3.12770 DOI: https://doi.org/10.24831/jai.v44i3.12770
Sciences, H. A. (2022). Morphological Characterization of Local Rice from Tanjung Buka Village, Tanjung Palas Tengah District, Bulungan Regency. 6(6): 1–6.
Syafii, M., S. Purwoko, B., S. Dewi, I., & B. Suwarno, D. W. 2018. Agronomic Characteristics of Dihaploid Rice Lines Originated by Anther Culture from Three-Way Crosses. Indonesian Journal of Agronomy, 46(1): 9–16. https://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/16187
https://doi.org/10.24831/jai.v46i1.16187 DOI: https://doi.org/10.24831/jai.v46i1.16187
Ulum, B. 2018. Adaptability Test of Various Lines and Varieties of Upland Rice (Oryza sativa L.) on Acidic Soils.