Growth, Components, and Yield Capacity of 14 Rice (Oryza Sativa L.) Genotypes by Anther Culture
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Abstract
Background. Anther culture techniques have great potential for increasing productivity, quality, and competitiveness of crop commodities through anther culture techniques to obtain pure double haploid lines.
Aims. Through breeding activities, it is hoped that a variety of new superior lines can be produced, which in addition to having high productivity, also possess several other characteristics that support efforts to improve quality and competitiveness.
Methods. The experiment was conducted in Lengkong Village, Bojongsoang District, Bandung Regency. The daily temperature range during the experiment was between 16ºC and 29ºC, and the relative humidity ranged from 60% to 95%. Rainfall during the study varied between 48.26 mm - 256.54 mm. Temperature, relative humidity, and rainfall at the experimental location were in the optimal range for rice cultivation. The genetic material used was 14 rice genotypes consisting of 12 dihaploid (DH) lines from IPB anther culture and 2 comparison varieties, namely Bioni 63 Agritan Ciherang and Inpari 18. The lines tested were FS3-15-2-2, FS3-29-1-2, FS3-36-3-1, FS3-47-1-1, FS3-60-1-2, FS3-67-2-1, FS4-13-2-1, FS4-27-2-1, FS4-27-3-1, FS4-34-1-1, FS4-41-1-2, FS442-2-2.
Conclusion The test results showed: 1) There were differences in growth characteristics, yield components, and yield capacity of 14 rice genotypes (12 anther cultures and 2 control varieties. 2) The twelve tested lines had the same yield capacity as the Bioni63 Ciherang Agritan variety and were superior to the Inpari 18 variety.
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References
Afifah, Z., Carsono, N., Sari, S., & Anas, A. 2020. Yield Testing and Selection of F4 and F6 Generation Rice Families from Crosses of Sintanur x PTB 33 and Pandanwangi x PTB 33 in Jatinangor. Agrosainstek: Journal of Agricultural Science and Technology, 4(1), 28–34. https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/96.
https://doi.org/10.33019/agrosainstek.v4i1.96
Azmi, Y., B.S. Purwoko, I.S. Dewi, M. Syukur, and T. Suhartini. 2017. Anther Culture of Crossbreeding of Local Black Rice with Cultivated Varieties (Fatmawati and Inpari 13). J. Agron. Indonesia, December 2017, 45(3):228-234. ISSN 2085-2916 e-ISSN 2337-3652
http://jai.ipb.ac.id. DOI: https://dx.doi.org/10.24831/jai.v45i3.12544
Akbar, M., Fauzi, Asis, Asis, Lizmah, Fitria, & Sumeinika. 2022. Relationship between Agronomic Characteristics of Ciherang and Inpari 32 Rice Varieties in Rainfed Lowland Areas. Jurnal Agrium, 19(1):29. https://ojs.unimal.ac.id/agrium/article/view/6764. https://doi.org/10.29103/agrium.v19i1.6764
Akbar, M. R., Purwoko, B. S., Dewi, I. S., Suwarno, W. B., & Sugiyanta, D. 2019. Determination of Selection Index for High-Yielding Rainfed Lowland Rice Dihaploid Lines. Indonesian Journal of Agronomy, 47(2): 111–118. https://doi.org/10.24831/jai.v47i2.25032
Anshori, M. F., Purwoko, B. S., Dewi, I. S., Ardie, S. W., Suwarno, W. B., & Safitri, D. H. 2018. Heritability, Characterization, and Clustergram Analysis of Dihaploid Rice Lines Resulting from Anther Culture. Indonesian Journal of Agronomy, 46(2): 119. https://doi.org/10.24831/jai.v46i2.18377
Carsono, N., Lidiasari, D., Sari, S., & Wicaksana, N. 2022. Use of Natural and Synthetic Putrescine for Callus Induction and Anther Culture Regeneration of Four Rice Genotypes. Agriculture, 32(3): 314. https://doi.org/10.24198/agrikultura.v32i3.36784
Dewi, I.S., S. Azmy, B.S. Purwoko. 2024. Callus induction and plant regeneration from anther culture of gamma rays irradiated rice. AIP Conf. Proc. 2957, 080017 (2024). https://doi.org/10.1063/5.0183941. https://pubs.aip.org/aip/acp/article-abstract/2957/1/080017/3262246/Callus-induction-and-plant-regeneration-from
Feng, Zhizun, Huangwei Chu, Liming Cao, Ruiyun Wang, Anpeng Zhang. 2026. A Review of Research Progress in Rice Anther Culture. Molecular Plant Sciences, Mol. Biol. 2026 48(1):18. https://www.mdpi.com/1467-3045/48/1/18. https://doi.org/10.3390/cimb48010018.
Gunarsih C., B.S. Purwoko, I.S. Dewi, W.B. Suwarno, & Nafisah. 2022. Evaluation of Dihaploid Lowland Rice Lines Resulting from Anther Culture Using Index Selection. Indonesian Journal of Agronomy, 50(1):18–25.
https://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/39898. https://doi.org/10.24831/jai.v50i1.39898
Iriani, Y.F., D.C. Prayantini, H.N. Nahampun, R. Mastuti, N. Harijati. 2025. The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.). Journal of Science Education Research 11(8): 38-45. DOI: 10.29303/jppipa.v11i8.11884. https://jppipa.unram.ac.id/index.php/jppipa/article/view/11884
Lantos, C., M. Jansco, A. Szekely, E. Nagy, T. Szaloki and J. Pauk. 2022. Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rize (Oryza sativa L.) Breeding. Plants 11(24), 3446. https://doi.org/10.3390/plants11243446. https://www.mdpi.com/2223-774
Linton, J. D., Klassen, R., Jayaraman, V., Walker, H., Brammer, S., Ruparathna, R., Hewage, K., Thomson, J., Jackson, T., Baloi, D., Cooper, D. R., Hoejmose, S. U., Adrien-Kirby, A. J., Sierra, L. A., Pellicer, E., Yepes, V., Giunipero, L. C., Hooker, R. E., Denslow, D., Anane, A. (2020). Agronomic Performance and Yield Potential of Several Dihaploid Rice (Oryza sativa L.) Lines from Anther Culture in Karawang Agronomic Regency. Sustainability (Switzerland), 14(2) : 1–4. http://www.unpcdc.org/media/15782/sustainable procurement practice.pdf
Mawaddah, Purwoko, B. S., Dewi, I. S., & Wirnas, D. D. 2018. Characterization of Agronomic Traits of Dihaploid Red Rice Plants with High Yield Potential Obtained through Anther Culture. Indonesian Journal of Agronomy, 46(2): 126-132. P-ISSN: 2085-2916, E-ISSN: 2337-3652. https://doi.org/10.24831/jai.v46i2.16249
Firdaus, M.F., Bambang Sapta Purwoko, Iswari Saraswati Dewi, & Willy Bayuardi Suwarno. 2022. Physicochemical Characterization of Dihaploid Black Rice Lines. Indonesian Journal of Agronomy, 50(1): 1–9. https://doi.org/10.24831/jai.v50i1.39850
Prasetia, A. A., Jazilah, S., & Badrudin, U. 2022. The Effect of Planting Systems on the Growth and Production of Several Rice Varieties (Oryza sativa L.). Biofarm: Agricultural Scientific Journal, 18(1): 53.
https://doi.org/10.31941/biofarm.v18i1.1887
Romero, F.M. and A.G. Arias. 2019. CRISPR/Cas9: Development and Application in Rice Breeding. Rice Science 26(5): 265–281. ScienceDirect. Elsevier. https://www.sciencedirect.com/science/article/pii/S1672630819300599?via%3Dihub
https://doi.org/10.1016/j.rsci.2019.08.001
Safitri, H., B. Purwoko, I. Dewi, S.W. Ardie. 2016. Anther Culture to Obtain Salinity-Tolerant Rice Lines. J. Agron. Indonesia 44 (3): 221–227. https://www.researchgate.net/publication/316205586_Kultur_Antera_untuk_Mendapatkan_Galur_Padi_Toleran_Salinitas. DOI: 10.24831/jai.v44i3.12770
Sciences, H. A. (2022). Morphological Characterization of Local Rice from Tanjung Buka Village, Tanjung Palas Tengah District, Bulungan Regency. 6(6): 1–6.
Syafii, M., S. Purwoko, B., S. Dewi, I., & B. Suwarno, D. W. 2018. Agronomic Characteristics of Dihaploid Rice Lines Originated by Anther Culture from Three-Way Crosses. Indonesian Journal of Agronomy, 46(1): 9–16. https://journal.ipb.ac.id/index.php/jurnalagronomi/article/view/16187
https://doi.org/10.24831/jai.v46i1.16187
Ulum, B. 2018. Adaptability Test of Various Lines and Varieties of Upland Rice (Oryza sativa L.) on Acidic Soils.