RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung

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Published: May 7, 2026
DOI: https://doi.org/10.62885/agrosci.v3i3.1161
Keywords:
Bats, Braja Harjosari, Coronavirus, RNA Isolation

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Vidyanti Kurniasih
University of Lampung
Elly Lestari Rustiati
University of Lampung
Priyambodo Priyambodo
University of Lampung
Eko Agus Srihanto
Lampung Disease Investigation Center
Enny Saswiyanti
Lampung Disease Investigation Center
Dian Neli Pratiwi
Akar Lestari Indonesia, Bandar Lampung

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks.  The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). 


Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park.


Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii.  RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution.


Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.

Article Details

How to Cite
Kurniasih, V., Rustiati, E. L., Priyambodo, P., Srihanto, E. A., Saswiyanti, E., & Pratiwi, D. N. (2026). RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung. Jurnal Agrosci, 3(3), 263–268. https://doi.org/10.62885/agrosci.v3i3.1161
Issue
Vol. 3 No. 3 (2026): Vol 3 No 3 January 2026
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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