Sex Determination on Racing Pigeons (Columba livia) Molecularly Using Blood Samples
Article Sidebar
Main Article Content
Abstract
Background. Pigeons (Columba livia) belong to the Columbidae. Pigeons possess diverse ecological, economic, and aesthetic values, as they are monomorphic, exhibiting similar characteristics between male and female individuals, especially at a young age.
Aims. In collaboration with the Lampung Disease Investigation Center, a study has been done to confirm the sex determination of pigeons at a young age by the PCR technique. Blood samples were taken in two locations, East Lampung and Kota Metro. Molecular análisis was carried out at the Biotechnology Laboratory, Lampung Disease Investigation Center.
Methods. The technique includes DNA extraction, DNA amplification, electrophoresis and visualization. Result. It poses a significant challenge for pigeon breeders in sex determination. Blood is a source of genetic material that can be used to determine the sex of birds. The Chromo-Helicase-DNA-binding Protein (CHD) gene is one of the genes used as a marker to molecularly differentiate the sex of birds. The sex chromosomes in female individuals have heterozygous chromosomes (ZW) and male individuals have homozygous sex chromosomes (ZZ).
Conclusion. Of eleven pigeon blood samples, there were seven female and four male individuals, and there was one mistake in the gender prediction by the pigeon owner
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
El Islami, S. I., Purwaningrum, M., and Haryanto, A. 2021. Molecular Sex Determination of Masked Lovebird (Agapornis personata) by Polymerase Chain Reaction Method. Proceedings of KOBI 2nd International Confer, 1(1): 48-53.
Elvanda, D. 2023. Planninginformation on the maintenance and care of racing pigeons through e-book media. Indonesian Computer University: Bandung.
Eprilurahman, R., Asti, H. A., Hadisusanto, S., Yudha, D. S., Trijoko., Ramadani, R. S., Pranoto, F. S. and Muhtianda, I. A. 2018. The Richness of Fauna of Gianyar, Bali: Shrimp, Fish, Amphibians, Reptiles, Birds and Mammals. Gadjah Mada University Press : Yogyakarta.
Fitriana, F., Resita, R., Disastra, Y., Alfatik, G. H., Artdita, C. A., Haryanto, A., and Aziz, F. 2022. Comparison of five primary types of polymerase chain reaction to accurately identify the sexes of birds of the Order Columbidae. Journal of Veterinary Science, 40(2): 205-220.
Fitrian, Y. 2014. Feather-BasedCHDZ and CHDW Gene Identification Id in Parakeets (Melopsittacus undulatus) to Determine Sex by PCR (Polymerase chain reaction) Method. Airlangga University: Surabaya.
Fridolfsson AK and Ellegren H. 1999. A simple and universal method for molecular sexing of non-ratite birds. Journal of Avian Biology. 30(1):116-121.
Harvey, M. G., Bonter, D. N., Stenzler, L. M., and Lovette, I. J. 2006. A comparison of plucked feathers versus blood samples as DNA sources for molecular sexing. Journal of Field Ornithology, 77(2): 136-140.
Hikmatyar, M.F., Royani, J.I., Dasumiati. (2015). Isolation and Amplification of DNA in Rats (Thyponium flagelliform) for the identification of Genetic Diversity, Indonesian Journal of Biotechnology and Bioscience, 2(2), 42-48
Kadri, M. H. M., Septinova, D., and Riyanti, R. 2016. Characteristics and Behavior of Local High Pigeons Male and Female. Scientific Journal of Integrated Animal Husbandry, 4(2) : 156-160.
Literature, Y. 2021. Validation of Columbidae Family Bird Sex from Seller Information Compared to Polymerase Chain Reaction (PCR) and Necropsy. Gadjah Mada University: Yogyakarta.
Mustafa, H., Rachmawati, I., and Udin, Y. 2016. Measurement of DNA Concentration and Purity of Mosquito Genome. Journal of Disease Vectors, 10(1): 7-10.
Pratiwi, E., and Widodo, L. I. 2020. Quantification of gene extraction results as a critical factor for the success of Rt PCR examination. Indonesian Journal of Health Sciences, 4(1): 1-9.
Pratomo, Y. W., Zahida, F., and Yuda, P. 2021. Comparison of DNA isolation methods as PCR templates for molecular sex identification of Javanese Kettle (Charadriius javanicus) using Primer 2550F/2718R. Biota: Scientific Journal of Life Sciences, 6(2) : 78-86.
Setiani, N. A., Tritama, E., and Tresnawulansari, A. 2021. Optimization of Optical Density (OD) in Salmonella typhi Genome Isolation Using Genomic DNA Purification Kit. Indonesian Journal of Pharmaceutical Science and Technology, 10(1): 35-43.
Sidstedt, M., Hedman, J., Romsos, E. L., Waitara, L., Wadsö, L., Steffen, C. R., ... and Rådström, P. 2018. Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR. Analytical and bioanalytical chemistry, 410: 2569-2583.
Stevens, A.J., Taylor, M.G., Pearce, F.G. and Kennedy, M. 2017. Allelic dropout during polymerase chain reaction due to Gquadruplex structures and DNA methylation is widespread at imprinted human loci. G3 (Bethesda), 7(3): 1019-1025.
Syamsia, P. N. 2022. Analysis of Traded Birds in Bandar Jaya, Central Lampung Regency. University of Lampung : Bandar Lampung.
Yimtragool, N., and Changtor, P. 2022. Some Parts of the Feather Can be a Non-Invasive Genetic Sample for Sexing in Avians. Iranian Journal of Applied Animal Science, 12(1): 161-166